Insilico Analysis and Homology Modeling of Salutaridine Reductase of Papaver somniferum
نویسندگان
چکیده
Salutaridine reductase (NADPH) is a key enzyme involves in morphine biosynthesis. BLASTP search waer performed to found orthologous proteins across different plant species. Domain analysis of all orthologous proteins was performed using Pfam and it was observed that Adh_short domain was conserved across all proteins. 3D structure of Salutaridine reductase was not available in PDB database, therefore Homology model of Salutaridine reductase was constructed using HOMER. A template structure (PDB ID: 2PFG) was selected from protein databank (PDB) using BLASTP with BLOSUM62 sequence alignment scoring matrix. The protein model was validated using PROCHECK. The model structure was visualized and superimposed with template using Discovery Studio Visualizer (Accelrys) and Swiss PDBviewer.
منابع مشابه
Molecular modeling and site-directed mutagenesis reveal the benzylisoquinoline binding site of the short-chain dehydrogenase/reductase salutaridine reductase.
Recently, the NADPH-dependent short-chain dehydrogenase/reductase (SDR) salutaridine reductase (E.C. 1.1.1.248) implicated in morphine biosynthesis was cloned from Papaver somniferum. In this report, a homology model of the Papaver bracteatum homolog was created based on the x-ray structure of human carbonyl reductase 1. The model shows the typical alpha/beta-folding pattern of SDRs, including ...
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